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Decreased lipin 1β expression in visceral adipose tissue is associated with insulin resistance in polycystic ovary syndrome

B Mlinar, Department of Clinical Biochemistry, Faculty of Pharmacy, Ljubljana, Slovenia
M Pfeifer, Department of Endocrinology, Diabetes and Metabolic Diseases, University Medical Centre Ljubljana, Ljubljana, Slovenia
E Vrtacnik Bokal, Department of Obstetrics and Gynaecology, University Medical Centre Ljubljana, Ljubljana, Slovenia
M Jensterle, Department of Endocrinology, Diabetes and Metabolic Diseases, University Medical Centre Ljubljana, Ljubljana, Slovenia
J Marc, Department of Clinical Biochemistry, Faculty of Pharmacy, Ljubljana, Slovenia

Correspondence: Barbara Mlinar, Email: barbara.mlinar{at}ffa.uni-lj.si

Abstract

Objective: In polycystic ovary syndrome (PCOS), insulin resistance (IR) appears with high prevalence and represents the major cause of cardiometabolic complications. Lipin 1β regulates lipid metabolism and augments insulin sensitivity. The impact of lipin 1β expression in visceral and subcutaneous adipose tissue of PCOS patients on IR was studied for the first time.

Methods: Eighty-five PCOS patients and 44 controls were enrolled for subcutaneous tissue biopsy, out of which 25 patients and 30 controls underwent also visceral adipose tissue biopsy. Gene expression of lipin 1β, peroxisome proliferator-activated receptor , lipoprotein lipase, hormone-sensitive lipase, adiponectin, and glucose transporter 4 in subcutaneous and visceral adipose tissue was measured along with markers of obesity, IR and PCOS.

Results: In PCOS patients, lipin 1β expression in both adipose depots was decreased compared to controls: 0.76 (0.67-0.84) vs. 1.16 (0.90-1.43) for visceral, and 0.91 (0.73-1.10) vs. 1.30 (1.03-1.57) for subcutaneous depot (for both p<10-4). The difference remained significant after adjustment for BMI and also when comparing only lean patients with lean controls. In PCOS patients, visceral adipose lipin 1β expression negatively correlated with HOMA-IR (r=-0.474, p=0.017), BMI (r=-0.511, p=0.009), and waist circumference (r=-0.473, p=0.017), waist circumference remaining significant (p=0.027) in multiple regression. Subcutaneous lipin 1β expression in PCOS correlated negatively with BMI, waist circumference and plasma triglycerides, and positively with HDL-cholesterol. Subcutaneous, but not visceral lipin 1β expression, correlated positively with the studied genes

Conclusions: Lipin 1β appears to be involved in the pathogenesis of IR in PCOS.