HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Raspé, E Articles by Ludgate, M Search for Related Content PubMed Articles by Raspé, E Articles by Ludgate, M

Identification of the thyroid Na⁺/I^– cotransporter as a potential autoantigen in thyroid autoimmune disease

Raspé E, Costagliola S, Ruf J, Mariotti S, Dumont JE, Ludgate M. Identification of the thyroid Na⁺/I^– cotransporter as a potential autoantigen in thyroid autoimmune disease. Eur J Endocrinol 1995;132: 399–405. ISSN 0804–4643

The thyroid gland is the target of several autoimmune diseases. Specific thyroid proteins have been identified as autoantigens associated with these diseases (e.g. thyroperoxidase, thyroglobulin and the thyrotrophin (TSH) receptor). In this paper, we report that the serum of a patient suffering from Hashimoto's thyroiditis, autoimmune gastritis and rheumatoid arthritis was able to inhibit the chronic TSH-induced I^– uptake of dog thyrocytes in culture, even at a 1:1000-fold dilution, without affecting their ⁸⁶Rb⁺ uptake. This blocking activity is rare as 147 sera (from patients positive for antibodies to the thyroid microsomes and the gastric parietal cell antigen, patients with Sjögren's syndrome, patients with a high titre of microsomal antibodies and low or negative for antibodies to thyroperoxidase, and patients with a high titre of microsomal antibodies and frank hypothyroidism) were negative when tested for their ability to inhibit I^– uptake. Subsequently we tested 20 murine monoclonal antibodies previously obtained by immunizing mice with a crude human thyroid membrane preparation, which were all negative when tested against thyroglobulin and thyroperoxidase. One of the monoclonal antibodies displayed a 50% inhibition of the chronic TSH-induced ¹²⁵I^– uptake of dog thyrocytes without affecting the ⁸⁶Rb⁺ uptake of the cells. Immunoglobulins purified from the ascite fluid by affinity chromatography on a protein A cellulose column had the same characteristics. Taken together, the data suggest that thyroidal ¹²⁵I^– uptake can be inhibited by antibodies, that autoantibodies in the patient's serum are most probably responsible for the observed inhibition and therefore that the Na⁺/I^– cotransporter is probably an autoantigen.

E Raspé, IRIBHN, School of Medicine, Free University of Brussels (ULB), 808 route de Lennik, 1070 Bruxelles, Belgium

This article has been cited by other articles:

				O. Dohan, A. De la Vieja, V. Paroder, C. Riedel, M. Artani, M. Reed, C. S. Ginter, and N. Carrasco The Sodium/Iodide Symporter (NIS): Characterization, Regulation, and Medical Significance Endocr. Rev., February 1, 2003; 24(1): 48 - 77. [Abstract] [Full Text] [PDF]

				J. Seissler, S. Wagner, M. Schott, M. Lettmann, J. Feldkamp, W. A. Scherbaum, and N. G. Morgenthaler Low Frequency of Autoantibodies to the Human Na+/I- Symporter in Patients with Autoimmune Thyroid Disease J. Clin. Endocrinol. Metab., December 1, 2000; 85(12): 4630 - 4634. [Abstract] [Full Text]

				A. De la Vieja, O. Dohan, O. Levy, and N. Carrasco Molecular Analysis of the Sodium/Iodide Symporter: Impact on Thyroid and Extrathyroid Pathophysiology Physiol Rev, July 1, 2000; 80(3): 1083 - 1105. [Abstract] [Full Text] [PDF]

				R. A. Ajjan, E. H. Kemp, E. A. Waterman, P. F. Watson, T. Endo, T. Onaya, and A. P. Weetman Detection of Binding and Blocking Autoantibodies to the Human Sodium-Iodide Symporter in Patients with Autoimmune Thyroid Disease J. Clin. Endocrinol. Metab., May 1, 2000; 85(5): 2020 - 2027. [Abstract] [Full Text]

				M. T. Kilbane, R. A. Ajjan, A. P. Weetman, R. Dwyer, E. W. M. McDermott, N. J. O’Higgins, and P. P. A. Smyth Tissue Iodine Content and Serum-Mediated 125I Uptake-Blocking Activity in Breast Cancer J. Clin. Endocrinol. Metab., March 1, 2000; 85(3): 1245 - 1250. [Abstract] [Full Text]

				C. Spitzweg, W. Joba, K. Schriever, J. R. Goellner, J. C. Morris, and A. E. Heufelder Analysis of Human Sodium Iodide Symporter Immunoreactivity in Human Exocrine Glands J. Clin. Endocrinol. Metab., November 1, 1999; 84(11): 4178 - 4184. [Abstract] [Full Text]

				C. Spitzweg, W. Joba, W. Eisenmenger, and A. E. Heufelder Analysis of Human Sodium Iodide Symporter Gene Expression in Extrathyroidal Tissues and Cloning of Its Complementary Deoxyribonucleic Acids from Salivary Gland, Mammary Gland, and Gastric Mucosa J. Clin. Endocrinol. Metab., May 1, 1998; 83(5): 1746 - 1751. [Abstract] [Full Text]

				R. A. Ajjan, C. Findlay, R. A. Metcalfe, P. F. Watson, M. Crisp, M. Ludgate, and A. P. Weetman The Modulation of the Human Sodium Iodide Symporter Activity by Graves' Disease Sera J. Clin. Endocrinol. Metab., April 1, 1998; 83(4): 1217 - 1221. [Abstract] [Full Text]