HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ogawa, K. Articles by Hayashi, Y. Search for Related Content PubMed Articles by Ogawa, K. Articles by Hayashi, Y.

Effect of streptozotocin injection on expression of immunoreactive foilistatin and βA and βB subunits of inhibin/activin in rat pancreatic islets

Ogawa K, Ono K, Kurohmaru M, Hayashi Y. Effect of streptozotocin injection on expression of immunoreactive follistatin and βA and βB subunits of inhibin/activin in rat pancreatic islets. Eur J Endocrinol 1995;132:363–9. ISSN 0804–4643

The effect of streptozotocin (STZ) on expression of immunoreactive follistatin and β-subunits of inhibin/activin in pancreatic islets was studied by immunohistochemistry. To localize immunoreactive follistatin in pancreatic islets, two different anti-follistatin sera were used. Anti-follistatin(300–315) serum detects only long-form follistatin, while anti-follistatin(123–134) serum detects both long- and C-terminal truncated short-form follistatin in sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotting. Although an intense reaction with anti-follistatin(123–134) was found in pancreatic B cells, a reaction with anti-follistatin(300–315) was not present in any type of cells. This result suggests that only short-form follistatin is expressed in pancreatic islets. It was confirmed that immunoreaction with anti-β_A(1–10)-Tyr serum was found in pancreatic B cells. The anti-β_B(1–10)-Tyr reaction was intense in A cells but weak in B cells. These findings suggest that the expression pattern of each β-subunit differs in each type of islet cells. At 2 weeks after STZ injection (65 mg/kg), insulin immunoreaction still remained in B cells, although most pancreatic B cells were destroyed. In contrast, immunoreactions of follistatin and β-subunits in B cells disappeared 24 h after STZ treatment. These results indicate that STZ affects the production of follistatin or β-subunits of inhibin/activin in pancreatic B cells rather than insulin secretion.

Kenji Ogawa, Department of Veterinary Anatomy, Faculty of Agriculture, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

This article has been cited by other articles:

				S. La Rosa, S. Uccella, S. Marchet, C. Capella, and R. V. Lloyd Localization of Inhibins and Activins in Normal Endocrine Cells and Endocrine Tumors of the Gut and Pancreas: an Immunohistochemical and In Situ Hybridization Study J. Histochem. Cytochem., February 1, 2004; 52(2): 217 - 225. [Abstract] [Full Text] [PDF]

				T. Morioka, H. Koyama, H. Yamamura, S. Tanaka, S. Fukumoto, M. Emoto, H. Mizuguchi, T. Hayakawa, I. Kojima, K. Takahashi, et al. Role of H1-Calponin in Pancreatic AR42J Cell Differentiation Into Insulin-Producing Cells Diabetes, March 1, 2003; 52(3): 760 - 766. [Abstract] [Full Text] [PDF]

				Y.-Q. Zhang, H. Zhang, A. Maeshima, H. Kurihara, J.-i. Miyagawa, T. Takeuchi, and I. Kojima Up-Regulation of the Expression of Activins in the Pancreatic Duct by Reduction of the {beta}-Cell Mass Endocrinology, September 1, 2002; 143(9): 3540 - 3547. [Abstract] [Full Text] [PDF]

				C. Demeterco, G. M. Beattie, S. A. Dib, A. D. Lopez, and A. Hayek A Role for Activin A and Betacellulin in Human Fetal Pancreatic Cell Differentiation and Growth J. Clin. Endocrinol. Metab., October 1, 2000; 85(10): 3892 - 3897. [Abstract] [Full Text]

				D. J. Hecht, A. J. Davis, C. F. Brooks, and P. A. Johnson Molecular Cloning and Expression Analysis of the Complementary Deoxyribonucleic Acid for Chicken Inhibin/Activin {beta}B Subunit Biol Reprod, May 1, 2000; 62(5): 1128 - 1134. [Abstract] [Full Text]