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Follicle-stimulating hormone regulation of inhibin -subunit mRNA in staged rat seminiferous tubules

Kaipia A, Penttilä T-L, ToppariJ. Follicle-stimulating hormone regulation of inhibin -subunit mRNA in staged rat seminiferous tubules. Eur J Endocrinol 1994;131:323–9. ISSN 0804–4643

Steady-state levels of inhibin- and -β_B mRNAs are higher in stages II–VI of the seminiferous epithelial cycle than in stages VII–VIII We investigated follicle-stimulating hormone (FSH) regulation of inhibin -subunit mRNA in stages II–VI and VII–VIII to study whether the stage specificity is due to differential hormonal regulation by FSH. Follicle-stimulating hormone caused a significant increase of inhibin- mRNA levels in both stages during a 20-h incubation. The mechanism of the FSH effect was studied further in stages VII-VIII. Maximal stimulation of the inhibin- mRNA level was achieved with 100 µg/l FSH, dibutyryl-3',5'-cyclic adenosine monophosphate (db-cAMP, 0.2 mmol/l) and Spadenosine-3',5'-monophosphothionate (Sp-cAMPS, 10 µmol/l) (a cAMP agonist). The presence of RpcAMPS (200 µmol/l) (a cAMP antagonist) abolished the stimulation, Rp-cAMPS alone had no effect. inhibin-β_B mRNA levels in stages VII–VIII were not affected by FSH, db-cAMP, Sp-cAMPS or Rp-cAMPS. Phorbol 12-myristate 13-acetate (100 nmol/l) had no effect on inhibin- or -βB mRNA levels. Actinomycin D abolished the stimulatory effect of FSH on inhibin- mRNA expression. In conclusion, FSH stimulated inhibin- mRNA expression similarly both in stages II–VI and VII–VIII of the seminiferous epithelial cycle and the stimulation in stages VII–VIII was cAMP-mediated.

Jorma Toppari, University of Turku, Institute of Biomedicine, Department of Physiology, Kiinamyllynkatu 10, FIN-20520 Turku, Finland